Lactoferrin, Isolation,Purification and Antimicrobial Effects
نویسندگان
چکیده
Lactoferrin (Lf) is an 80 kDa iron-binding glycoprotein with multifunctional properties and useful for clinical and commercial applications. Currently, Lf has some advantages including, immune system modulation, antibacterial activity and as antioxidant in infant and adult of human as well as animal health. In the present study, antibacterial activity of Lf has been scrutinized after isolation and purification from cow’s milk colostrums against Pseudomonas aeroginosa. Lf purified using CMsephadex C50, a cation exchange chromatography. Bacterial samples were isolated from scald patients and microbial activity was confirmed by biochemical tests. Different concentration of Lf (0, 200, 400, 500, 600 and 700 μg/ml) treated on Pseudomonas colonies as well as E.coli( DH5α) as positive control for two days. Our result indicated LF was effective on Pseudomonas growth and the least and the inset inhibitory concentration were 400,and 700 μg/ml, respectively. The result showed that The Lf has more strong effect than other previous studies.
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جداسازی و خالص سازی لاکتوفرین از شیر گاو
Background and purpose: Lactoferrin is a multifunctional protein, which is useful for clinical and commercial applications. Recently it has been shown that ingestion of Lactoferrin has advantages including: immune system modulation, anti-microbial activity and antioxidant in neonates and adults in both animals and humans. The present study has been conducted with the purpose of isolation and op...
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